RESUMO
ABSTRACT PURPOSE: To evaluate the effects of photobiomodulation therapy (PBMT) at 685 nm on diabetic wound healing in rats suffering from bacterial infection induced by Staphylococcus aureus (S. aureus). METHODS: Thirty streptozotocin-induced diabetic rats were allocated into two groups: control and PBMT. A 4-cm full-thickness linear-incision was made on the dorsal midline and was contaminated with S. aureus. The wounds in the PBMT group were irradiated daily for 5 consecutive days, starting 3 days after the induction and always in the mornings. RESULTS: The result revealed that PBMT resulted in a significant decrease in S. aureus CFU in the PBMT group in comparison to the control group (P<0.05). The length of wounds, in the 2nd and 3rd weeks, in the PBMT group were significantly shorter compared to the control group (P<0.05). PBMT caused a significant increase in the histological parameters in comparison to the control group (P<0.05). Moreover, PBMT significantly increased the breaking strength of the surgical scars produced in the skin of the PBMT group when compared to the control group (P<0.05). CONCLUSION: Photobiomodulation therapy may be useful in the management of wound infection through a significant bacterial growth inhibition and an acceleration of wound healing process.
Assuntos
Animais , Masculino , Ratos , Infecções Estafilocócicas/radioterapia , Infecção da Ferida Cirúrgica/radioterapia , Cicatrização/efeitos da radiação , Terapia com Luz de Baixa Intensidade/métodos , Diabetes Mellitus Experimental/complicações , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos da radiação , Infecção da Ferida Cirúrgica/microbiologia , Distribuição Aleatória , Ratos Wistar , Diabetes Mellitus Experimental/induzido quimicamente , Modelos Animais de DoençasRESUMO
PURPOSE: To characterize the effects of low-level laser (LLL) on third-degree burn wounds which were infected with Staphylococcus aureus (S. aureus) in diabetic rats. METHODS: Thirty streptozotocin-induced diabetic rats were divided into two groups: the control and the LLL groups. Third-degree burns were induced using a heated metal rod, and then, were contaminated with S. aureus. The wounds in the LLL group were irradiated with a LLL (685nm) daily for five consecutive days, starting three days after the induction. The wound area was measured at 3, 5, 8, 14 and 21 days after burning. At the end of trial, the skin samples were harvested. RESULTS: Reduction in wound areas in the LLL and control groups were significantly different only on the 21st day (p<0.05). The mean bacterial numbers in the LLL group were significantly lower (p<0.05) than those in the control group. The number of macrophages, new blood vessels, fibroblast, and elevated collagen deposition in the LLL group significantly increased compared to the control group (p<0.05). The mean breaking strength of scars in the control group was significantly lower (p<0.05) than that of the LLL group. CONCLUSION: The low-level laser improved the healing of S. aureus third-degree burn infections in diabetic rats.
Assuntos
Animais , Masculino , Infecções Estafilocócicas/radioterapia , Staphylococcus aureus , Queimaduras/microbiologia , Queimaduras/radioterapia , Terapia com Luz de Baixa Intensidade/métodos , Diabetes Mellitus Experimental/fisiopatologia , Staphylococcus aureus/efeitos da radiação , Fatores de Tempo , Queimaduras/patologia , Distribuição Aleatória , Reprodutibilidade dos Testes , Resultado do Tratamento , Ratos WistarRESUMO
Assuntos
Animais , Masculino , Ratos , Terapia com Luz de Baixa Intensidade/métodos , Staphylococcus aureus Resistente à Meticilina/efeitos da radiação , Infecções Estafilocócicas/radioterapia , Cicatrização/efeitos da radiação , Infecção dos Ferimentos/radioterapia , Análise de Variância , Contagem de Colônia Microbiana , Modelos Animais de Doenças , Staphylococcus aureus Resistente à Meticilina/crescimento & desenvolvimento , Doses de Radiação , Distribuição Aleatória , Ratos Wistar , Estatísticas não Paramétricas , Infecção dos Ferimentos/microbiologiaRESUMO
A infecçäo de enxertos de prótese vascular apresenta uma mortalidade de 60-100%. Os antibióticos têm pouco efeito nessa complicaçäo. Trabalhos anteriores utilizaram radioterapia no tratamento de gangrena gasosa. Esta investigaçäo apresenta o resultado do uso de 1000 rads no tratamento de enxertos vasculares de Dacron infectados com S. aureus, no cäo. A alta mortalidade sugere que a radiaçäo suprime a medula óssea. Uma radiaçäo menor pode ser necessária para tratar as complicaçöes sépticas.